The authors said that the expected heterozygosity was higher than the noticed heterozygosity in contrast of what offered in Table 2 (which misses the indices of Lamu inhabitants). E.g. the variety of landraces from Kwale, Kilifi and Lamu assigned to cluster 1, and so on. As well as, figures (Fig. 3 and Fig. 5) have been blindly introduced, only codes of cashew landraces have been offered without corresponding populations, difficult to identify clusters 1, 2, three and their composition for each forms of markers. Figures 2 and 3, depicting population construction and admixture, ought to have clear legends and be simple to interpret. Pace helps ensure that figures meet PLOS necessities. 6. PLOS authors have the option to publish the peer evaluation historical past of their article (what does this mean? ). This should replicate the implication of these genetic indices on the background and breeding historical past of cashew landraces already highlighted. Since solely local cashew landraces had been used, the admixture must be robust enough if some other cashew varieties (external for instance as reference) had been used to analyse whether the Kenyan landraces are actually purely indigenous for the purpose of conservation or breeding. 13. Interpretation of Findings: The dialogue interprets the outcomes effectively, however linking the findings to practical implications for breeding packages might be elaborated.
However, suggesting specific future research directions based mostly on the outcomes would enhance this part. However, while, the examine could be very attention-grabbing and contribute to the body of science, an extra thorough improvement is required. Since the study appeared at the usefulness of DArT markers in genomic studies, related findings and applicability weren't mentioned with different types of markers used in previous studies on genetic diversity in cashew plant and/or in other plants in the country or region to scrutinize their suitability and success. The research was carried out to analyse the genetic variety and population structure of cashew plants from three counties specifically Kwale, Kilifi and Lamu in Kenya using two types of makers: SilicoDArT and SNP markers. In discussions, it was stated that "the use of a dual-marker strategy, incorporating both silicoDArT and SNP markers elevates the precision and reliability of genetic variety assessments capturing numerous sorts of variations within individual cashew populations". However, extra context on the importance of genetic diversity studies in cashew could strengthen the rationale. However, providing extra detail on the software program variations and particular packages utilized in R would enhance transparency. However, discussing potential causes for the noticed admixture and gene movement in more element would add depth.
The main reasons behind are as follow: 1) it failed to be presented within the really useful structure and written in a complete way to make sure readers understand the research. Referring to outcomes, cashew landraces in Kenya are reasonably divergent demonstrating that they are intently related thought they were separated into 2-3 sub-populations based on the inhabitants structure. Referring to Table 1, the outcomes are clear. 3) Results offered had been incomplete and mismatched. Page 7 L three - 5: for clarity (because the goal was to find out the genetic range), the sentence ought to change as a result of the genetic parameters offered have been decided to point the level of genetic variety. 3. The interpretation of results was not enough to make sure readers understand the main key elements in the subject similar to genetic diversity and inhabitants construction. 12. Genetic Relationships: The results on genetic relationships and inhabitants construction are vital. Are there occurrences of controlled crossing? P eight L 12: is there the difference between the subpopulations mentioned on the identical page L 8? P12, L11 2, three or 4 subpopulations? Conclusions should be drawn by additionally considering the important thing findings corresponding to relationship between landraces (ancestral subpopulations and three subpopulations), admixture and the potential fixation of alleles revealed by the heterozygosity.
To make sure readers perceive the connection between cashew landraces from the three populations (Kwale, Kilifi and Lamu), the membership of cashew landraces should be indicated in two subpopulations formed primarily based on cross-entropy and three subpopulations formed based on Q matrix and PCoA. Low variance between Lamu and different counties and excessive variance inside cashew landraces from Lamu don't clarify the exception of Lamu county, since should you verify the variance between cashew landraces from Kilifi and Kwale is also low and the variance inside populations is excessive as shown by AMOVA. If not, should the "subpopulation" in L 12 be modified into populations (original populations: Kilifi, Kwale and Lamu)? These findings (He and Ho) have been additionally offered in no right subsection "markers’ characterisation" and are usually not the identical as those presented in Table 2. The authors should also clarify why no genetic indices of cashew landraces from Lamu have been calculated. 6. Sampling Strategy: The sampling technique is described, however extra element on the stratified random system and why it was chosen would enhance readability. Subsequently, they need to clearly clarify why they needed to perform one other inhabitants structure analysis.
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